GSM6672400: Smad2 - MCF10A; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMAD2
Cell type
Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease
Attributes by original data submitter
Sample
source_name
MCF10A
cell line
MCF10A
cell type
Normal human breast epithelial cell
chip antibody
Smad2 (CST, #5339)
treatment
TGF-beta (-)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM6672400
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed using the SimpleChIP Enzymatic Chromatin IP kit (9003, Cell Signaling Technologies), according to the manufacturer's instructions. The antibodies used for ChIP were anti-H3K4me3 (9751), anti-H3K27c (8173), anti-Smad2 (5339), and anti-c-Jun (9165), which were purchased from Cell Signaling Technologies. ChIP-seq libraries were prepared using the KAPA HyperPrep Kit.